A Montreal couple accused of killing three of their daughters and the man's first wife have lost a bid to maintain some contact with three of their other children.

Mohammed Shafia and his wife, Tooba Mohammed Yahya, sat quietly as they appeared in a Kingston, Ont., bail court on Tuesday.

A court order prohibiting the pair from communicating with each other or their eldest son and co-accused, 18-year-old Hamed Shafia, remains in effect.

The son was not in court with his parents, but is scheduled to make a bail court appearance by video-link on Friday.

All three are charged with four counts of first-degree murder and matching counts of conspiracy to commit murder in the deaths of three of the family's daughters, 19-year-old Zainab Shafia, 17-year-old Sahar, 13-year-old Geeti and 50-year-old Rona Amir Mohammed.

Their bodies were pulled from the Rideau Canal near Kingston on June 30 after a car was discovered submerged near one of the locks.